Protein test with Bicinchoninic acid

 

 

 

Solution

 

 A

1 % (w/v) BCA = (2,2'-Bichinolyl-4,4'-dicarbonic acid) Na₂-salt

           2 % (w/v) Na₂CO₃.H₂O

           0,16 % (w/v) Na₂-tartrate

           0,4 % (w/v) NaOH

           0,95 % (w/v) NaHC0₃

 

 adjust to pH 11.25 with NaOH or NaHCO₃

 

 B

4 % CuSO₄.5H₂0

 

 C

100 Vol. A + 2 Vol. B

 

• Calibration curve with 1 mg/ml BSA  0 - 100 µg.
• Sample or standard 100 µl + 2,0 ml C.
• 30 min incubation at 37°C
• Quantify in photometer at 562 nm.