Cleavage of N-terminal Modification

 1.       AcSer und AcThr 

Agent :                      wf. TFA, 1h 40°C

Reaction :                 Rearrangement of N-Ac to O-Ac

2.       N-formyl 

Agent :                      0.6 n HCl, 24h 25°C

Reaction :                 Deformylation 

3.       Pyroglutamate 

Agent :                     Pyroglutamate-Aminopeptidase

                                5µg / 100 µl sample sol.

                                50 mM Na-P pH=7, 10 mM DTT,

                                10h 37°C

Reaction :                 Cleavage 

4.      N-Acetylamino-Aa 

Agent :                     Acylamino acid-releasing-enzyme

                                (only peptide < 20AS in solution!)

                                first modify N-terminal Aa with PITC

                                then 100 µl + 50 mU in

                                200 mM Na-P pH=7.2, 1 mM DTT,

                                12h 37°C

Reaction :                 Cleavage of modified terminal Aa