DNA via diphenylamine

A

           5% Perchloric acid (w/v)

B

           300 mg diphenylamine in 20 ml acetic acid +

           0,3 ml conc. sulfuric acid and

           0,1 ml 50%iger aqu. acetaldehyde.

           (fresh !!)

 

 

• The sample is mixed with 1,0 ml of A (up to 1:1)
• The mixture is heated to 70 °C for 15 minutes.
• 1,0 ml with about 5 - 10 µg desoxyribose (30 - 60 µg DNA) is mixed with 2,0 ml of solution B and incubated over night at 30 °C.
• Avoid evaporation!!
• DNA is quantified spectrophotometrically at 600 nm
• Using desoxyribose as standard 1 µg desoxyribose = 6 µg DNA.