ELISA

 

1.    Incubate coated plate with diluted sample in ELISA-buffer for 2 h at 37 °C

2.    Wash 3 times with phosphate buffered saline

3.    Add enzyme labeled antibody and incubate for 2 h at 37°C

4.    Wash 3 times with phosphate buffered saline

5.    Add enzyme substrate and incubate  for10 minutes at RT

6.    Stop reaction with e.g. diluted sulfuric acid

7.    Measure absorbance with an ELISA-plate reader