How to measure diffusion
Principle : eximer forming
The ratio of excimer and monomer - emission includes the diffusion coefficient
A lipid membrane can be treated as if it is a grid with well defined positions about 0.8 nm apart. The number of exchange processes between adjacent positions within such a grid to form an excited dimer is proportional to the diffusion of the molecule within the membrane.
D = ¼ . nj . l2
nj = jump frequency
l = mean jump distance
