Protein quantitation via UV-absorption

 

A) Formula due to WARBURG and CHRISTIAN

 mg protein / ml = 1,55 A₂₈₀^1cm - 0.76 A₂₆₀^1cm

 

B) Formula due to KALCKAR and SHAFRAN

 mg protein / ml = 1.45 A₂₈₀^1cm - 0.74 A₂₆₀^1cm

 

C) Formula due to WHITAKER and GRANUM

 mg protein / ml = (A₂₃₅^1cm -  A₂₈₀^lcm) : 2.51

 

D) Formula for immune globulins (lgG)

 mg IgG / ml = A₂₈₀^1cm : 1.38

 

 

LAMBERT-BEER’S LAW

 

E=log₁₀[I_o/I] = e.c.d

 

Wavelength [nm]