Mass spectroscopy:

Types :

 

1. Angle-resolving MS

2. often as Tandem-MS

3. TOF = Time of flight - MS

 

Detection :

 

* mostly M⁺ - Ions ( sometimes H⁺, Na⁺, K⁺,..)

* for specific applications M^-

 

c of sample:

 

5 - 100 pmol / µl

 

Matrix :

 

No or nearly no volatile ions, thus:

Alcohols, Water, Acetonitrile, acetic acid,..

 

Volume :

 

1 - 100 µl

 

Application :

 

Molecular mass

Sequence of peptides and Oligos

Post-translat. modifications of proteins

N- und C-terminal modifications