SPR

 

System :                   e.g. BIAcore from Pharmacia

Flow :                       1-100 µl/min

Time/assay :            5-10 min

Detection limit :        depends on molecular size !!

                                 e.g. IgE 1 nM and theophylline 4.5 µM !

Application :              Interaction analysis

                                 Epitop-Mapping,..

 

Setup of the sensor chip: