From

T Celsius

Monacylglycerides

dichloromethane/ethanol/water (40:20:1)

18

slow cooling from 40 C to 18 C

dichloromethane/ethanol/water (3:2:0.15)

20

Clear supernatant was kept in a sealed tube at 20 C over a period of several weeks.

water-free diethylether-ethanol (10:1)

Crystals are highly hygroscopic and were handled in a dry nitrogen atmosphere.

flash chrom. (5% boric acid/silica gel, hexane /ethyl acetate = 95:5)

42.5

single crystals obtained in ethyl acetate by slow evaporation at 4 C

petroleum spirit

20-40

crystals are biaxial positive & frequently twined on the planes.

dichloromethane/ethane/water

< 8

crystal are in needles.

dichloromethane/ethane/water

20

crystals are thin plates

Diacylglycerides

ether/ethanol/water

14

Crystals redissolved above 22 C. Flocculent precipitate. Monoclonic crystals with two molecules in the asymmetric unit

diethyl ether/ethanol/water (10:1:0.05)

12

Minor impurities removed by diethyl ether/ethanol soln. Needle-like single crystals

a solution in hexane

Crystal obtained by slow evaporation of the solvent

dichloromethane/methane (2:1)

Induced by vapor diffusion of diethylether. Thin lanceolate crystals

ethyl acetate

4

Flash chromatography and slow evaporation

Triacylglycerides

petroleum spirit

40-60

purified by chromatography on aluminium oxide column

petroleum ether

39.4-53.2

in thick plates. Crystals are triclinic