Organelle Markers

Antibodies and enzymes are useful for locating membranes in preparations and by microphotography techniques. Enzymes can be tested via their catalytic activity. Other proteins are identified via antibodies supplied by e.g. StressGen (complete line of antibodies). For conjugations: alkaline phosphatase (AP), horseradish peroxidase (HRP), biotin, and fluoroscein isothiocyanate (FITC) are employed.

PROTEIN

Endoplasmic Reticulum - Lumen

ERp57, ERp72, Glucosidase II, Grp58, Grp78 (BiP), Grp94, Histone H1, Hsp25, Hsp27, Hsp47 (Colligin), c-Jun, KDEL, Membrin, PDI, TAP1, UGGT

Endoplasmic Reticulum - Membrane

rbet1, Calnexin, HO-1, HO-2, p450 Reductase, Tapasin

Endosome - Early

Rab4, Rab5, Syntaxin 13

Endosome - Late

Rap1

Golgi - Cis

GS28, KDEL Receptor, Membrin (GS27), rbet1

Golgi - Medial

Membrin (GS27)

Golgi - Trans

Syntaxin 6

Lysosome

Lamp1, Lamp2, Rap1

Mitochondria - Matrix

Cpn10, Grp75 (mtHsp70), Hsp60, Superoxide Dismutase (Mn)

Mitochondria - Membrane

Bcl-2, Bcl-w

Nucleus

c-Jun, Estrogen Receptor, NF Kappa B (c-Rel), NF Kappa B (Rel A), PARP, Progesterone Receptor, Thyroid Hormone Receptor

Plasma Membrane - Membrane

CD27, CD40, CD40L, CD45, Fas/CD95, TNF-R1

Plasma Membrane - Peripheral

FADD, Grb2, RAIDD, Ras

Plasma Membrane - Neuromuscular Junction

Agrin

Plasma Membrane - Synaptic Terminal - Membrane

Syntaxin 1

Plasma Membrane - Synaptic Terminal - Peripheral

Bassoon, GKAP, PSD95, SAP97, SAP102, rSec6, rSec8, SNAP-25

Synaptic Vesicles - Membrane

SV2A, SV2B, Synaptogyrin, Synaptophysin, Synaptotagmin, VAMP (Synaptobrevin)

Synaptic Vesicles - Peripheral

Amphiphysin, AP180, CaMKII, Cysteine String Protein (CSP), Dynamin, Rab3A, Synapsin I, Synapsin II